Biology - Microscopy Practical

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Why is iodine solution added to onion cells?
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To stain the cells so they are easier to see.
Why is coverslip lowered at an angle?
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To prevent air bubbles from forming.
Which objective lens should you start with?
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The lowest power objective (usually 4x)
Which focusing knob should be used first?
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The coarse adjustment knob.
Which focusing knob is used for clear focus?
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The fine adjustment knob.
What should you do after finding cells under low power?
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Switch to a higher power lens and refocus using the fine adjustment knob.
How is total magnification calculated?
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Total magnification=eyepiece lens x objective lens
What should the biological drawings include?
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Labels and magnification.
What is the formula for calculating actual size?
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Actual size=image size/magnification
How many micrometres are in 1mm?
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1000 micrometres
Put the osmosis practical steps in order: Record final mass, Place in solutions, Cut potato cylinders, Blot dry, Record initial mass
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1. Cut potato cylinders 2. Record initial mass 3. Place in solutions 4. Blot dry 5. Record final mass
Put these microscope steps in order: Use fine focus, Place slide on stage, Choose low power lens, Use coarse focus, Switch to higher power
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1. Place slide on stage 2. Choose low power lens 3. Use coarse focus 4. Use fine focus 5. Switch to higher power
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